REAL SCIENCE

Abstracts/Posters/Spoken Presentations

Gebert, T., Edward, J., Son, S., & King, M. (2024, June 13–17). Immunomodulatory effects of Bacillus subtilis and Weizmannia coagulans on HT-29-mtx cell line (Poster presentation). ASM Microbe, Atlanta, GA, United States.

Leyva-Jimenez, H., Galbraith, E. A., Delago, F., Doden, H. L., Soto, C., & Dirks, B. (2024, Jan 30–Feb 1). A DNA-based microbial surveillance platform can provide insights on bacterial interactions leading to coinfections in broiler chickens (Technical talk). International Production and Processing Expo, Atlanta, GA, United States.

Pederson, K., Galbraith, E., Lee, J., Son, S., & King, M. (2024, Feb 24–27). A multi-year quantitative surveillance of nursery pigs reveals increasing carriage of F18+ Escherichia coli (Poster presentation). 55^th Annual American Association of Swine VeterinariansMeeting, Nashville, TN, United States.

Woodward, A., Spencer, J. D., Galbraith, E., & Arnold, B. (2024, Feb 24–27). The effects of fungal and bacterial infections and their toxin metabolites on pig health and performance and the impacts of a Bacillus-based mitigant in the presence of complex disease syndromes (Spoken presentation). 55^th Annual American Association of Swine Veterinarians Meeting, Nashville, TN, United States.

Publications

Leistikow, K. R., May, D. S., Suh, W. S., Vargas Asensio, G., Schaenzer, A. J., Currie, C. R., & Hristova, K. R. (2024). Bacillus subtilis-derived peptides disrupt quorum sensing and biofilm assembly in multidrug-resistant Staphylococcus aureus. mSystems 0:e00712-24. https://doi.org/10.1128/msystems.00712-24

Goetz, B. M., Abeyta, M. A., Rodriguez-Jimenez, S., Opgenorth, J., McGill, J. L., Fensterseifer, S. R., Arias, R. P., Lange, A. M., Galbraith, E. A., & Baumgard, L. H. (2024). Effects of a multistrain Bacillus-based direct-fed microbial on gastrointestinal permeability and bio-markers of inflammation during and following feed restriction in mid-lactation Holstein cows. Journal of Dairy Science, Feb 21, S0022-0302(24)00502-2. https://doi.org/10.3168/jds.2023-24352

Patents

King, M. R. & Son, S. (2024) Microorganisms for waste treatment. Chinese Patent No. ZL 201880085135.9. China National Intellectual Property Administration.

King, M. R., Son, S., Leistikow, K., & Augspurger, N. R. (2024). Direct-fed microbials. Canadian Patent No. 3,016,203. Canadian Intellectual Property Office.

King, M. R., Son, S., Leistikow, K., & Spencer, J. D. (2024). Microbials for feed. U. S. Patent No. 11,944,656 A1. U.S. Patent and Trademark Office.

King, M. R., Son, S., Galbraith, E., Fensterseifer, S. R., & Arias, R. P. (2024). Microbials for animals. European Patent No. 3860360. European Patent Convention.

Patent Applications

Duersteler, M., Son, S., & King, M. (2024). Microorganism compositions for enhancing soil quality and plant growth. U.S. Patent Application No. 63/621,387. U.S. Patent and Trademark Office.

Select Invited Speaker Presentations

Leistikow, K. (2024). Exploring nature's arsenal: Unconventional antimicrobial sources and next-gen discovery strategies (Invited speaker presentation). ASM Microbe, Atlanta, GA, United States.

Leistikow, K. (2024). Introduction to the microbiome: Impacts, benefits, and challenges in healthy and diseased animals (Invited speaker presentation). 55^th Annual American Association of Swine Veterinarians Meeting, Nashville, TN, United States.

Webinars

Cray, J. (2024). Creating quality microbial products (Webinar). Microbial Discovery Group.

Menako, J. (2024). Managing FOG in wastewater (Webinar). Microbial Discovery Group.

Abstracts/Posters/Spoken Presentations

Chapa, V., Duersteler, M., Menako, J., Romanek, D., Son, S., & King, M. (2023, June 15–19). A multi-strain Bacillus blend increased sludge digestion in a pulp and paper wastewater treatment facility (Poster presentation). ASM Microbe, Houston, TX, United States.

Fensterseifer, S. R., Arias, R. P., Goetz, B. M., Abeyta, M. A., Rodriguez-Jimenez, S., Opgenorth, J., Freestone, A. D., Lange, A., Galbraith, E., & Baumgard, L. H. (2023, June 25–28). Bacillus-based direct-fed microbial alters fecal parameters during feed restriction in mid-lactation Holstein cows (Abstract). American Dairy Science Association Annual Meeting, Ottawa,Ontario, Canada. Journal of Dairy Science, 106(suppl. 1), 295.

Galbraith, E. A., Lange, A. L., Delago, F., Haag, D., Staggs, K., Son, S., & King, M. R. (2023, Jan 24–26). Insights from high- and low-performing broiler flocks revealed by a DNA-based microbial surveillance platform (Technical talk). International Production and Processing Expo, Atlanta, GA, United States.

Galbraith, E. A., & Leyva-Jimenez, H. (2023, Mar 8–10). PathKinex™, a microbial surveillance tool, quantifies poultry pathogens and demonstrates control using Novela® ECL (Technical talk). ViVAsia, Bangkok, Thailand.

Lee, J., Spencer, J., Galbraith, E., Son, S., & King, M. (2023, Mar 4–7). DFM Pak® improves manure characteristics to influence environmental and health benefits in the pit, the pen, and in the air (Spoken presentation). 54^th Annual American Association of Swine Veterinarians Meeting, Aurora, CO, United States.

Patents

King, M., Son, S., & Heile, C. (2023). Microorganisms for waste treatment. U.S. Patent No. 11,634,554 B2. U.S. Patent and Trademark Office.

King, M., Son, S., Lange, A., Duersteler, M., & Galbraith, E. (2023). Microorganisms for plant pathogen inhibition. U.S. Patent No. 11,627,741 B2. U.S. Patent and Trademark Office.

Patent Applications

Leistikow, K., & Hristova, K. (2023). Probiotic derived compounds that inhibit biofilm formation and improve antibiotic sensitivity against Staphylococcus aureus and uses thereof. Publication No. 63,469,776.

Leistikow, K., Son, S., & King, M. (2023). Microbials and antibiotics. U.S. Patent Application No. 20230346854 A1. U.S. Patent and Trademark Office.

Select Invited Speaker Presentations

Menako, J. (2023, Sept 5–7). Los productos de biorremediación de calidad conducen a resultados medibles consistentes y una major calidad del agua (Quality bioaugmentation products lead to consistent water quality and measurable results) (Invited speaker presentation). AquaTech Mexico, Mexico City, Mexico.

Menako, J. (2023, Sept 5–7). Utilización de la bioaumentación en beneficio de los sistemas de aguas residuales (Using bioaugmentation to benefit wastewater treatment systems through the reduction of H2S, sludge, fat, oil, and grease, and improving water quality) (Invited speaker presentation). AquaTech Mexico, Mexico City, Mexico.

Menako, J. (2023, Oct 10). Improving wastewater treatment systems using bioaugmentation (Invited speaker presentation). WWOA—Southern District Regional Operators’ Meeting, Potosi, WI, United States.

Menako, J. (2023, Oct 23–26). Productos de biorremediación de calidad que generan resultados medibles, constantes y mejoran la calidad del agua (Quality bioremediation products lead to consistent measurable results and better water quality) (Invited speaker presentation). Aqua Expo Guayaquil, Guayaquil, Ecuador.

Menako, J. (2023, Nov 6–9). Benefits of bioaugmentation in wastewater (Invited speaker presentation). AquaTech Amsterdam, Amsterdam, Netherlands.

Webinars

Cray, J. (2023). Leveraging the strength of microbial-based cleaning products in the industrial, institutional, and consumer markets (Webinar). Microbial Discovery Group.

Menako, J., & Chapa, V. (2023). Solutions for pulp and paper facilities: Decreasing costs and reducing sludge with bioaugmentation (Webinar). Microbial Discovery Group.

Menako, J. (2023). Why bioaugmentation paired with micronutrients improves water quality & leads to measurable results (Webinar). Microbial Discovery Group.

Abstracts/Posters/Spoken Presentations

Fensterseifer, S. R., Arias, R. P., Peter, C. M., Lange, A., & Galbraith, E. (2022, June 19–22). Effects of a Bacillus-based direct-fed microbial on health and production of high-performing lactating dairy cows (Abstract). American Dairy Science Association Annual Meeting, Kansas City, MO, United States. Journal of Dairy Science, 105(suppl. 1), 338–339.

Lange, A. M., Galbraith, E. A., Arias, R. P., Fensterseifer, S. R., Son, S., & King, M. R. (2022, June 19–22). Microbial virulence genes in rectal swab samples are correlated with environmental, metabolic, and gut health stressors in U.S. dairy cows (Abstract). American Dairy Science Association Annual Meeting, Kansas City, MO, United States. Journal of Dairy Science, 105(suppl. 1), 44.

Lee, J., Galbraith, E., Krahn, G., Spencer, J., Son, S., & King, M. (2022, Feb 26–Mar 1). DFM Pak®, a novel combination of Bacillus strains reduces environmental E. coli bioburden, manure buildup, and odor compounds in the swine housing environment (Poster presentation). 53^rd Annual American Association of Swine Veterinarians Meeting, Indianapolis, IN, United States.

Leistikow, K., Solis, E., Marshall, C., & Hristova, K. (2022). Long-term exposure to Bacillus subtilis derived compounds increases phenotypic sensitivity of Staphylococcus aureus to multiple antibiotics (Poster presentation). ASM Microbe, Washington, D.C., United States.

Publications

Cray, J. (2022). Bacillus-based bioaugmentation reduces sludge in wastewater lagoons. Wastewater Digest. https://www.wwdmag.com/wastewatertreatment/article/21438349/bacillus-based-bioaugmentation-reduces-sludge-in-wastewaterlagoons

Cray, J., Duersteler, M., Menako, J., Romanek, D., Son, S., & King, M. (2022). Reducing hydrogen sulfide production within municipal collection systems using bioaugmentation. Water Environment Federation.

Leistikow, K. R., Beattie, R. E., & Hristova, K. R. (2022). Probiotics beyond the farm: Benefits, costs, and considerations of using antibiotic alternatives in livestock. Frontiers in Antibiotics. https://doi.org/10.3389/frabi.2022.1003912

Leyva- Jimenez, H., Shen, S., McCormick, K., Martin, M., Liu, P., Haag, D., Galbraith, E., & M. Blair. (2022). Evaluation of a proprietary direct-fed microbial as a strategy to reduce hydrogen sulfide emissions from poultry excreta using a practical monitoring method. Journal of Applied Poultry Research, 31(1), 100231. https://doi.org/10.1016/j.japr.2021.100231

Zuckermann, F. A., Husmann, R., Chen, W., Roady, P., Pfeiff, J., Leistikow, K. R., Duersteler, M., Son, S., King, M. R., & Augspurger, N. R. (2022). Bacillus-based direct-fed microbial reduces the pathogenic synergy of a coinfection with Salmonella enterica serovar choleraesuis and porcine reproductive and respiratory syndrome virus. Infection and Immunity, 90(4), e00574-21. https://doi.org/10.1128/iai.00574-21

Patent Applications

King, M., Son, S., Heile, C., & Duersteler, M. (2022). Microorganisms for treatment of waste, water, or soil or for feeding to animals. U.S. Patent Application No. 20220289607 A1. U.S. Patent and Trademark Office.

Leistikow, K., Son S., & King, M. (2022). Microbial strains for virus inhibition. U.S. Patent Application No. 20220143109 A1. U.S. Patent and Trademark Office.

Select Invited Speaker Presentations

Cray, J., & Menako, J. (2022, May 2–5). Bioaugmentation: Lagoon sludge and collection system H₂S reduction (Invited speaker presentation). 41^st Annual Colorado Rural WaterAssociation Conference, Loveland, CO, United States.

Menako, J. (2022, Sept 6–8). Reducción de H2S y lodos mediante la bioaumentación de microorganismos (Bacillus lagoon sludge and collection system H2S reduction) (Invited speaker presentation). AquaTech Mexico, Mexico City, Mexico.

Menako, J. (2022, Sept 21–24). Reducing hydrogen sulfide within municipal collection systems using bioaugmentation (Invited speaker presentation). Association of Water Technologies Conference, Vancouver, British Colombia, Canada.

Abstracts/Posters/Spoken Presentations

Galbraith, E., Leistikow, K., Son, S., Lee, J., & King, M. (2021, Feb 27–Mar 2). Surveillance of microbial genes in over 5000 pigs reveals associations between gene abundance, health status and production stage (Poster presentation). 52^nd Annual American Association of Swine Veterinarians Meeting, Virtual.

Hensch, M., Miller, J., Spencer, J., & Lee, J. (2021, Feb 27–Mar 2). Optimizing gut health in the face of endemic enterics (Spoken presentation). 52^nd Annual American Association of Swine Veterinarians Meeting, Virtual.

Leistikow, K., Schmaling, G., Zingelman, K., & Hristova, K. (2021). Antimicrobial compounds produced by Bacillus subtilis inhibit Staphylococcus aureus biofilm attachment and growth (Poster presentation). World Microbe Forum, Virtual.

Shen, S., Leyva-Jimenez, H., McCormick, K., Haag, D., & Galbraith, E. (2021, July 19–22). Remediation of hydrogen sulfide emissions from poultry excreta by dietary supplementation of a proprietary microbial product (Abstract). 110^th Annual Poultry Science Association Meeting, Virtual. Poultry Science, 100(suppl. 1), 77.

Patents

King, M., Son, S., Augspurger, N., Spencer, J., Lange, A., & Engelhardt, M. (2021). Direct-fed microbials and methods of their use. U.S. Patent No. 10,905,135 B2. U.S. Patent and Trademark Office.

King, M., Son, S., Leistikow, K., & Augspurger, N. (2021). Direct-fed microbials. U.S. Patent No. 11,110,134 B2. U.S. Patent and Trademark Office.

Patent Applications

Leistikow, K., Edward, J., Son, S., & King, M. (2021). Microbials for feed. U.S. Patent Application No. 20210236563 A1. U.S. Patent and Trademark Office.

Select Invited Speaker Presentations

Leistikow, K. (2021). Back to BAC: Re-evaluating the therapeutic potential of Bacillus subtilis Against multidrug resistant Staphylococcus aureus biofilms (Invited speaker presentation). Milwaukee Microbiology Seminar Series.

Abstracts/Posters/Spoken Presentations

Fensterseifer, S. R., Arias, R. P., Peter, C. M., Haag, D., Lange, A. M., & Galbraith, E. A. (2020, June 22–24). Effects of a Bacillus-based direct-fed microbial on high- and low-health calf herds (Abstract). American Dairy Science Association Annual Meeting, Virtual. Journal of Dairy Science, 103(suppl. 1), 72.

Lee, J., Galbraith, E., Spencer, J., Son, S., & King, M. (2020, Mar 7–10). DFM Pak®, a novel combination of Bacillus strains selected for corn fiber utilization and biofilm activity, shows beneficial impacts on environmental and storage characteristics of manure (Poster presentation). 51^st Annual American Association of Swine Veterinarians Meeting, Atlanta, GA, United States.

Publications

Lange, A. (2020). Insights from microbial surveillance in broiler flocks. Poultry World: Gut Health, 116-117. https://www.poultryworld.net/specials/insights-from-microbialsurveillance-in-broiler-flocks/

Abstracts/Posters/Spoken Presentations

Augspurger, N., Hofacre, C., & Edward, J. (2019, July 15–18). The effect of a Bacillus-based direct-fed microbial on broiler chickens subjected to a necrotic enteritis challenge (Abstract). 108^th Annual Poultry Science Association Meeting, Montreal, Quebec, Canada. Poultry Science, 98(suppl. 1), 247–248.

Fensterseifer, S. R., Arias, R. P., Galbraith, E. A., & Peter, C. M. (2019, June 23–26). Effect of a Bacillus-based direct-fed microbial on production, health, and reproduction in lactating dairy cattle: A meta-analysis (Abstract). American Dairy Science Association Annual Meeting, Cincinnati, OH, United States. Journal of Dairy Science, 102(suppl. 1), 324.

Galbraith, E. A., Lange, A. M., Son, S., Arias, R. P., Fensterseifer, S. R., Peter, C. M., & King, M. R. (2019, June 23–26). Abundance of microbial virulence genes in rectal swabs from U.S. dairy cows varies by stage of lactation (Abstract). American Dairy Science Association Annual Meeting, Cincinnati, OH, United States. Journal of Dairy Science, 102(suppl. 1), 322.

Lange, A. M., Galbraith, E. A., Son, S., Arias, R. P., Peter, C. M., & King, M. R. (2019, June 23–26). Microbial virulence gene abundance in rectal swabs from U.S. dairy cows with or without gastrointestinal disease symptoms (Abstract). American Dairy Science Association Annual Meeting, Cincinnati, OH, United States. Journal of Dairy Science, 102(suppl. 1), 322.

Leistikow, K., Son, S., & King, M. (2019, Mar 9–12). Meta-analysis of enteric pathogen genes identified in 122 swine flows across the United States (Poster presentation). 50^th Annual American Association of Swine Veterinarians Meeting, Orlando, FL, United States.

Leistikow, K., Spencer, J., Son, S., & King, M. (2019, Mar 9–12). Pathogen virulence factors present in sow represent potential health threats to piglet (Poster presentation). 50^th Annual American Association of Swine Veterinarians Meeting, Orlando, FL, United States.

Patents

King, M., Son, S., Augspurger, N., Spencer, J., Lange, A., & Engelhardt, M. (2019). Direct-fed microbials and methods of their use. U.S. Patent No. 10,357,046 B2. U.S. Patent and Trademark Office.

King, M., Son, S., Leistikow, K., & Augspurger, N. (2019). Direct-fed microbials. U.S. Patent No. 10,335,440 B2. U.S. Patent and Trademark Office.

Select Invited Speaker Presentations

Peter, C. M., Galbraith, E. A., Fensterseifer, S. R., & Arias, R. P. (2019, Mar 26–28). Stateris, a novel microbial solution for dairy cow health (Invited speaker presentation). Central Plains Dairy Expo, Sioux Falls, SD, United States.

Publications

Augspurger, N. R., Peterson, G. I., Son, S., McCormick, K., & King, M. (2018). The effect of a Bacillus subtilis-based direct-fed microbial feed additive on the lactation performance of sows and their offspring. Journal of Animal Science, 96(2), 187-188. https://doi.org/10.1093/jas/sky073.345

Patent Applications

King, M., Son, S., Lange, A., Edward, J., Veldkamp, A., & Spencer, J. (2018). Nucleic acid detection and quantification methods and compositions. U.S. Patent Application No. 20180298428 A1. U.S. Patent and Trademark Office.

Abstracts/Posters/Spoken Presentations

Evaluation of a direct fed microbial (DFM) compared to antibiotic growth promoters in male broiler growth parameters. 2017. Smith, K., R. Latham, N. Augspurger, B. Brown, M. King, S. Son, and J.T. Lee. Poult. Sci. 96(E-Suppl. 1):(Abstr.).

Lange, A., Son, S., & King, M. (2017, Jun 1–5). Use of FTA cards for diagnostic reverse transcriptase PCR to determine Vibrio harveyi hemolysin toxin gene expression in shrimp pond samples (Abstract). ASM Microbe, New Orleans, LA, United States.

Patent Applications

King, M., & Son, S. (2017). Cold temperature-resistant microbials and methods of their use. U.S. Patent Application No. 20170166466 A1. U.S. Patent and Trademark Office.

Select Invited Speaker Presentations

Galbraith, E. A. (2017, Jan 10–11). Impacting microbial ecology in dairy cows for health and performance benefits (Invited speaker presentation). JBS United Midwest Dairy Conference, East Lansing, MI, United States and Archbold, OH, United States.

Abstracts/Posters/Spoken Presentations

Augspurger, N. R., Spencer, J. D., Son, S., Ley, J. A., & King, M. R. (2016, Mar 14–16). Improved growth performance of nursery pigs fed diets supplemented with a Bacillus subtilis-based direct-fed microbial feed additive (Abstract). Midwest American Society of Animal Sciences Meeting, Des Moines, IA, United States. Journal of Animal Science, 94(suppl. 2), 74.

Abstracts/Posters/Spoken Presentations

Evaluation of a direct fed microbial (DFMs) on broiler growth performance and processing yield. 2015. Brown, K., H. Walters, J. Price, N. Augspurger, M. King, S. Son, and J.T. Lee. Poult. Sci. 94(E-Suppl. 1):214(Abstr.).

Son, S., Lange, A. M., & King, M. R. (2015, May 26–30). Use of FTA cards for quantitative PCR detection of Vibrio Harveyi and Vibrio Campbelli toxin genes and gene expression within shrimp pond water samples (Abstract). World Aquaculture Society Meeting, Jeju, Korea.

Abstracts/Posters/Spoken Presentations

Son, S., Lange, A., Behn, H., & King, M. (2014, Feb 10–12). Combining the use of Whatman FTA cards and diagnostic PCR assays to determine the pathogenic potential of shrimp water samples shipped overseas (Abstract). Aquaculture America 2014, Seattle, WA, United States.

Publications

Albert, R. A., Zitomer, D., Dollhopf, M., Schauer-Gimenez, A., Struble, C., King, M., Son, S., Langer, S., & Busse, H-J. (2014). Proposal of Vibrionimonas magnilacihabitans gen. nov., sp. nov., a curved Gram negative bacterium isolated from Lake Michigan water. International Journal of Systematic and Evolutionary Microbiology, 64(2), 613-2.

King, M., Son, S., Lange, A., Behn, H., & Bradley, J. (2014). Field diagnostic tools for pathogenic vibrios. Global Aquaculture Advocate, May/June, 40–42.

Select Invited Speaker Presentations

King, M. (2012). Bioaugmentation for municipal and industrial wastewater treatment application (Invited speaker presentation). Delhi Jal Water Board, New Delhi, India.

Abstracts/Posters/Spoken Presentations

Brown, K., Walters, H., Price, J., Augspurger, N., King, M., Son, S., & Lee, J. T. Evaluation of a direct fed microbial (DFMs) on broiler growth performance and processing yield (Abstract). International Production and Processing Expo.

Patent Applications

Microorganisms For Treatment of Hydrocarbons or Oil. M. King, S. Son, M. Duersteler. Microbial Discovery Group.

Direct Fed Microbials Using B. Coagulans. M. King, S. Son. Microbial Discovery Group.

Select Invited Speaker Presentations

King, M. How microbes will change our world: The big picture on little things in an evolving era. Pig production outside the box (Invited speaker presentation). SwineTalks.

King, M. Bioaugmentation and PATTON principles (Invited speaker presentation). Canadian Continuing Education Wastewater Training Seminar.

King, M. Novel Sewage Treatment Technologies (Invited speaker presentation). Delhi Jal Water Board, New Delhi, India.

Creating Novel Bioaugmentation and Bioremediation Technologies. Pollution abatement in Ganga basin. Bioremediation: Potential and Challenges. Kanpur, India.

Bioaugmentation (Invited speaker presentation). Kentucky Sewage Treatment Continuing Education Workshop. Lexington, Kentucky.

Webinars

King, M. Plant and animal crossover platforms (Webinar).

King, M., Woodward, A., & Krahn, G. The science behind mycotoxin mitigation (Webinar).